Serratia marcescens chitobiase is a retaining glycosidase utilizing substrate acetamido group participation
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منابع مشابه
Serratia marcescens chitobiase is a retaining glycosidase utilizing substrate acetamido group participation.
The stereochemistry of the reaction catalysed by Serratia marcescens chitobiase was determined by HPLC separation of the anomers of N-acetylglucosamine produced during the hydrolysis of p-nitrophenyl N-acetyl-beta-d-glucosaminide (PNP-GlcNAc). In the early stages of the reaction, the beta-anomer was found to prevail, whereas the alpha-anomer dominated at mutarotation equilibrium. This establish...
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Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks. The API 20E system has been used widely, but is not individually satisfactor...
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The nucleotide sequence of the proBA operon from a proline-hyperproducing mutant of Serratia marcescens was determined. Two base substitutions were found: one in theproBstructura1 gene, coding for y-glutamyl kinase (GK), and a second one in the promoter region of the operon. The former base substitution led to a change of the predicted amino acid at position 117 from an alanine to a valine in G...
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Serratia marcescens is one of the most effective bacteria for degradation of chitin, a 1,4-β-linked polymer of N-acetyl-β-Dglucosamine (GlcNAc). Chitinolysis by S. marcescens involves at least four enzymes and a chitin-binding protein. Studies of the enzymology and the structures of the enzymes provide insight in how a natural set of chitinolytic enzymes may be built up. S. marcescens chitinase...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1997
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3280945